"Illumina/SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)/20014279/1 Ea

二代測序 Illumina 貨號

二代測序 Illumina 貨號

產(chǎn)品編號： 20014279

美  元  價： $2500.00

品       牌： Illumina

產(chǎn)       地： 美國

公       司： Illumina, Inc.

Product Highlights:

The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing.

Sensitive and unbiased characterization of transcriptional signatures – Highly sensitive and reproducIBLe gene detection in varied individual cells

Comprehensive single-cell RNA sequencing workflow – Fully supported workflow developed in collaboration by technology innovators

Powerful next-generation sequencing integrated with simple data analysis – Proven Illumina sequencing combined with streamlined, user-friendly analysis software

Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.

Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.

Data Analysis

Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.

Species Details

This kit has been validated for human and mouse samples. The BaseSpace Single-Cell RNA-Seq App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.

Specifications:

System Compatibility NextSeq 550,HiSeq 2000,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Species Category Human,Mouse

Content Specifications Captures the coding transcriptome (3u0027 enriched), with strand-specific information

Method Whole-Transcriptome Sequencing,mRNA Sequencing

Technology Sequencing

Specialized Sample Types Single Cells

Unbiased, Cell-Size Agnostic RNA-Seq:

Mouse cell lines (A20, NIH3T3) and human cell lines (HEK, BJ) were processed using the SureCell WTA 3′Library Prep Kit. Consistently high numbers of detected genes demonstrates that recovery of transcripts is not limited by cell size.

Confident Single-Cell Transcript Identification:

A. BaseSpace-generated plots of the number of unique molecular identifiers (UMI), i.e. transcripts, assigned to the mouse/mm10 (red) and human/hg19 (blue) genomes for each cell barcode. Unique transcripts mapping to both mouse and human (purple) represent cell doublets. B. Cumulated fraction of unique transcripts assigned to cell barcodes (linear scale). The inflection point (red line) determines the number of barcoded cells detected in the sequencing run.

Confident Cell Population Identification:

A. PCA clustering of 1384 single cells sorted from a 1:1 ratio mixture of HEK 293 and NIH 3T3 cells, sequenced and analyzed with the Single-Cell RNA-Seq BaseSpace App. B. Cell populations cluster based on expression of the human (hg19) RPL13 gene.

Confident Cell Subpopulation Identification:

A. Analysis in the Single-Cell RNA-Seq BaseSpace App using the t-SNE algorithm of a mixture of NIH 3T3 and HEK 293 cells identifies a distinct subpopulation of cells. B. Cells color coded by gene expression of hg19 RPL 13 confirms the identity of the subpopulation as human.

Cell Cycle Analysis of Single Cells by RNA-Seq:

Analysis of cell cycle state using the Single-Cell RNA-Seq BaseSpace App is based on unique transcript counts of genes associated with each phase of the cell cycle, normalized by the total count for each cell. Expression is centered by the median and scaled by the median absolute deviation for each cell cycle." 2,500.00 盒 上海易匯生物科技有限公司，是一家集研發(fā)、銷售為一體的生物企業(yè)，公司專注于生命科學和生物技術(shù)領域，專業(yè)提供分子生物學、免疫學、生命科學基礎研究以及臨床檢測等諸多領域的試劑、耗材、儀器等各類產(chǎn)品及生物技術(shù)服務。公司目前已銷售 LKT Laboratories，MEDICOM（麥迪康），Biovision，ApexBio，阿拉丁，麥克林，TCI，Epigentek,Eaivelly,Abnova,GeneBrick等品牌。